New Step by Step Map For Salmeterol Xinafoate
Ultimately, 1 μCi of 86Rb was included to each lifestyle and incubation was continued at 37 °C for an extra 10 min. Reactions ended up terminated by washing the cells with ice-cold a hundred mM MgCl2 and cell lysates ended up prepared for liquid scintillation counting. The 86Rb counts in parallel cultures with ouabain have been subtracted